LOCUS       40924_27613        5590 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLCPVRGNR104 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Hashinaka K.
  TITLE     Synthetic Autonomous Vectors Based on Palindromic Sequences of 
            Parvovirus B19
  JOURNAL   Published Only in Database (2001)
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Hashinaka K.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2000) Kazuya Hashinaka, Miyazaki Medical College, 
            Department of Biochemistry; 5200 Kihara, Kiyotake, Miyazaki 
            889-1692, Japan (E-mail:hasinaka@post1.miyazaki-med.ac.jp, 
            Tel:81-985-85-0985, Fax:81-985-85-2401)
REFERENCE   3  (bases 1 to 5590)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5590)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2001)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2000) Kazuya Hashinaka, Miyazaki Medical College, Department
            of Biochemistry"; volume: " 5200 Kihara, Kiyotake, Miyazaki 
            889-1692, Japan (E-mail:hasinaka@post1.miyazaki-med.ac.jp, 
            Tel:81-985-85-0985, Fax"; pages: "81-985-85-2401"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          12..485
                     /mol_type="other DNA"
                     /label=synonym:Parvovirus B19
                     /note="synonym:Parvovirus B19"
                     /db_xref="taxon:10798"
                     /organism="Human parvovirus B19"
     source          3369..3846
                     /mol_type="other DNA"
                     /label=synonym:Parvovirus B19
                     /note="synonym:Parvovirus B19"
                     /db_xref="taxon:10798"
                     /organism="Human parvovirus B19"
     repeat_region   15..397
     polyA_signal    complement(502..635)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(863..1087)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(1118..1906)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(1922..2629)
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     repeat_region   3455..3837
     rep_origin      3930..4518
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4579..4600
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4615..4645
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4653..4669
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4677..4693
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4837..5493)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 ggatccatgc atggccaaat cagatgccgc cggtcgccgc cggtaggcgg gacttccggt
       61 acaagatggc ggacaattac gtcatttcct gtgacgtcat ttcctgtgac gtcacttccg
      121 gtgggcggga cttccggaat tagggttggc tctgggccag cgcttggggt tgccttgaca
      181 ctaagacaag cggcgcgccg cttgatctta gtggcacgtc aaccccaagc aagctggccc
      241 agagccaacc ctaattccgg aagtcccgcc caccggaagt gacgtcacag gaaatgacgt
      301 cacaggaaat gacgtaattg tccgccatct tgtaccggaa gtcccgccta ccggcggcga
      361 ccggcggcat ctgatttggt gtcttctttt aaattttagc gggctttttt cccgccttat
      421 gcaaatgggc agccatttta agtgttttac tataatttta ttggttagtt ttgtaacgaa
      481 ggtaccgaat tcctcgacgg tatacagaca tgataagata cattgatgag tttggacaaa
      541 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      601 tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa ctccagcatg
      661 agatccccgc gctggaggat catccagccg gcgaacgtgg cgagaaagga agggaagaaa
      721 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      781 acacccgccg cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag
      841 ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg
      901 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac
      961 ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca
     1021 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa
     1081 ggcacagtcg aggctgatca gcgagctcga attctcagaa gaactcgtca agaaggcgat
     1141 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     1201 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     1261 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     1321 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     1381 gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     1441 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     1501 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     1561 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     1621 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     1681 aaggaacgcc cgtcgtggcg agccacgata gccgcgctgc ctcgtcttgc agttcattca
     1741 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     1801 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     1861 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcgatagca ccagcaccgt
     1921 tgtacagttc atccatgcca tgtgtaatcc cagcagctgt tacaaactca agaaggacca
     1981 tgtggtctct cttttcgttg ggatctttcg aaagggcaga ttgtgtggac aggtaatggt
     2041 tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt ttgttgataa tggtctgcta
     2101 gttgaacgct tccatcttca atgttgtggc gggtcttgaa gttcactttg attccattct
     2161 tttgtttgtc tgccatgatg tatacattgt gtgagttata gttgtattcc aatttgtgtc
     2221 ccagaatgtt gccatcttcc ttgaagtcaa taccttttaa ctcgattcta ttaacaaggg
     2281 tatcaccttc aaacttgact tcagcacgtg tcttgtagtt gccgtcatct ttgaagaaga
     2341 tggtcctttc ctgtacataa ccttcgggca tggcactctt gaaaaagtca tgccgtttca
     2401 tatgatccgg gtatcttgaa aagcattgaa caccatagca cagagtagtg actagggtgg
     2461 gccatggaac aggcagtttg ccagtagtgc agatgaactt cagggtaagt tttccgtatg
     2521 ttgcatcacc ttcaccctct ccactgacag agaacttgtg gccgttaaca tcaccatcta
     2581 attcaacaag aattgggaca actccagtga agagttcttc tcctttgcta gccatttctt
     2641 gcgcgcccga tcctgaacgg cagaggttac ggcagtttgt ctctccccct tccgggagcc
     2701 accttcttct ccaaccgtcc cggtcgcgct ctcggcgctt ctgaggagag aactggctga
     2761 gtgacgccct ttatagattc gcccttgtgt cccgcccctt cctttcccgc cctcccttgc
     2821 gctacggggc cgcccgcacc ggcctacacg gagcgcgcgc ggcggagttg ttgacgctag
     2881 ggctccggct ccctggttgg gtgttctttc tgacgcgaca ggaggaggag aatgtcctgg
     2941 tcctgtcgtc ctcctttcgg gtttcccgtg cactcaaacc gaggacttac agaacggagg
     3001 ataaagttag gccattttta ctcagcttcg gagttcaggc tcatttttca gctaaagtct
     3061 ctcattagta tccccccaca cacatcggga aaatggtttg tcctacgcat cggtaatgaa
     3121 ggcggggccc ttcgggtcct ccggagcggg ttccgggggt ggggggaagg agggagggac
     3181 gggacgggcc tcgttcatga atattcagtt caccgctgaa tatgcataag gcaggcaaga
     3241 tggcgcgtcc aatcaattgg aagtagccgt tattagtgga gaggccccag gacgttgggg
     3301 caccgcctgt gctctagtag ctttacggag ccctggcgct cgatgttcaa gcccaagctt
     3361 tcgcgagctc gagatcttaa gattgtgtaa tatgtatcaa aatttagaaa aataaacatt
     3421 tattgtggtt aaaaaattat gttgttgcgc tttaaaaatt taaaagaaga caccaaatca
     3481 gatgccgccg gtcgccgccg gtaggcggga cttccggtac aagatggcgg acaattacgt
     3541 catttcctgt gacgtcattt cctgtgacgt cacttccggt gggcgggact tccggaatta
     3601 gggttggctc tgggccagct tgcttggggt tgacgtgcca ctaagatcaa gcggcgcgcc
     3661 gcttgtctta gtgtcaaggc aaccccaagc gctggcccag agccaaccct aattccggaa
     3721 gtcccgccca ccggaagtga cgtcacagga aatgacgtca caggaaatga cgtaattgtc
     3781 cgccatcttg taccggaagt cccgcctacc ggcggcgacc ggcggcatct gatttggcca
     3841 cgggcccgga tcctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4081 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4261 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4321 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4561 tggccttttg ctcacatgta atgtgagtta gctcactcat taggcacccc aggctttaca
     4621 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4681 aaacagctat gaccatgatt acgccaagct acgtaatacg actcactagc ttggcactgg
     4741 gccaagctga atttctgcca ttcatccgct tattatcact tattcaggcg tagcaccagg
     4801 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
     4861 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg
     4921 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatagt
     4981 gaaaacgggg gcgaagaagt tgtccatatt agccacgttt aaatcaaaac tggtgaaact
     5041 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     5101 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     5161 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     5221 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     5281 cgtatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     5341 atttttcttt acggtcttta aaaaggccgt aatatccaga tgaacggtct ggttataggt
     5401 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     5461 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     5521 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     5581 tcttacatct
//
